Re: 4-colour FACScalibur Q

From: Rick Arthur Bright (rbright@emory.edu)
Date: Tue Jun 19 2001 - 13:10:08 EST

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We use PerCp in Fl3, along with PE, FITC, and APC with no problems.

Rick


On Tue, 19 Jun 2001, Joseph Webster wrote:

>
> A question to anyone using 4-colour FACScalibur - what mix
> of fluorochromes are you using successfully?
>
> In particular, what dyes are you using in FL3 that do not
> spill over into FL4?
> I'm guessing PerCP and PE/TxRED, what else?
>
> I guess the other side of that question is significant too,
> what dyes ARE giving channel spillover problems?
>
> TIA, Joseph.
> --
> Joseph Webster, Flow Cytometry Facility
> Centenary Institute, Sydney AUSTRALIA.
>

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