>Hi everybody , >Can anyone recommend a microsphere kit for performing simple cell counts >on numerous samples using the FACS? BD's Truecount beads are only sold >along with a MAb. Our cells do not have to be labelled. Any simpe >alternative? (NB: our good ol' Coulter particle counter is dead) >Tx >Karim Hello Karim, I have to do absolute cell counts here sometimes: I take a bottle of flow check beads (Beckman-Coulter) and wash them in FCS X2 then resuspend them at 1exp6/ml in FCS. Then you just spike your sample with them, the only thing that you have to take some care over is the initial count; I count ~1000 beads on a haemocytometer. If you don't need them sterile you can add ~1.0% azide. Regards Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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