Mikayla, you should look over and check thoroughly your lysing procedure. It may be aggregates of unlysed erythrocytes. Kind regards Anna At 16:40 2001-06-06 -0400, you wrote: > Dear cytometry community, These cells are also CD3, 4, 8, and 19 >negative, appear to be CD43 negative, and are not NK cells. It may be >helpful to know that these samples had very poorly defined populations in >the forward vs. side scatter plot. I have not yet tried erythrocyte or >platelet stains to rule out aggregates or nucleated red blood cells, nor >have I tried staining isolated mononuclear cells. Thank you in >advance. Sincerely, Mikayla Kob kob@cbr.med.harvard.edu Anna Porwit Hematopathology Lab. Department of Pathology, Radiumhemmet Karolinska Hospital, 171 76 Stockholm, Sweden Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:22 EST