Heather (and others . . .),
Not a specific response to your query, but here to suggest that you search the group's
archive . . .
< http://www.cyto.purdue.edu/hmarchiv/index.htm >
The question of analyzing frozen cells has been discussed several times - - you will
probably find lots of pertinent messages in the archive while waiting for new responses
. . .
MAK.
--
Mark A. KuKuruga, Managing Director
University of Michigan Flow Core
7416 CCGC 0946
(734) 647-3216, fax (734) 936-7376
kukuru@umich.edu
>>> "Heather Medbury" <Heather_Medbury@wsahs.nsw.gov.au> 05/30/01 08:05PM >>>
Hi FLOWers,
We are planning on doing intracellular cytokine staining, I saw in the BD application
note on 'Decting intracellular cytokines in activated lymphocytes' a brief comment
about being able to store stimulated samples at - 80°C and then stain at a later time.
Though we do plan on staining our samples straight away, it would be nice to store
the excess cells away.
Do any fellow FLOWers freeze their stimulated samples away for later analysis. If so is
it for intracellular staining or what other applications can this method be applied
to, and what is your freezing and subsequent staining protocol? what are the Pros /
cons etc.
Thankyou in advance,
Heather
Heather Medbury (PhD)
Senior Scientist
Department of Surgery
Westmead Hospital
Westmead 2145
61-2 9845 7677
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All experiments are preliminary
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"In his heart a man plans his course
but the Lord determines his steps"
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