----- Original Message ----- From: "Milton Maciel" <mmaciel@usp.br> To: cyto-inbox Sent: Wednesday, May 30, 2001 10:52 AM Subject: B-1? How? > Dear flow specialists, > > I work with a model of oral tolerance to mites and now I am looking for > IgA response in this model. One point that is interesting concerns about > B-1/B-2 cells along the protocol of oral tolerance induction. > I have addressed those cells in mesenteric lymph nodes and Peyer's > patches as well as in peritoneal cavity. My first question is what > markers are indicated to see the subsets. The second one is about Perc > cells: I find three or four populations as defined by complexity X size. > Someone has an idea in which population B-1 cells localize? > > PS. = I use CD 5 X IgA / B220 X IgA / CD23 X IgA > B220 X CD23 / B220 X CD 5 > B220 X Mac-1 (just to Perc cells) > I really would be very glad in receiving some suggestion and or new > strategies! > > Harmest regards, >
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