Dear Flowers, Hello all. I want some information regarding the expression (RNA not protein)of various apoptosis marker/proteins in human PBMC and at what time points does their expression switch on after stimulation (PMA/A23187) basically looking for the first detectable stage of apoptosis. I want to design and use some RTPCR primers so as to detect apoptosis markers at the transcription level. Does any one have an idea after how long of the mRNA transcription does the protein translation take place and when the apoptosis machineray is activated (say a time lag of 30min etc). Thanks a ton. Thanks a lot for your help for my previous query regarding cord blood. I think I had sent a summary of the responses but it does not seem to have reached the list. Thanks a lot anyway. Regards, Rana ____________________________________________________________________ Get free email and a permanent address at http://www.netaddress.com/?N=1
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