There is one thing we all seem to forget when looking at the drops profile on the screen. What we see is coming from the strobe light flashing synchronously with the drop drive frequency. How could we see superimposed low frequency of lets say 20-30Hz on the screen? Purity and recovery at the end of such sort would probably tell us if there was malicious interference. I did a few good sorts with continuous powerful wall-drilling next door. I guess that forces generating drops on the micro level are so huge that vibrations from the "big" world scaled down to this level wouldn't be anything near powerful to disturb the drops. Sasha --- David Coder <dcoder@u.washington.edu> wrote: > > Out here on the eastern edge of the Pacific Rim, there is the > potential for all > sorts of disturbances including such low frequency vibrations as > earthquakes. A > good example is the "event" of last February--a magnitude 6.8 > earthquake. In > addition to the normal building vibration on the third floor above > ground, there > was considerable building sway. Despite this, benchtop analyzers > continued > without a pause, and moreover, a sort in progress continued without a > change of > the droplet breakoff position. It's a long way saying that the > vibration > isolation of most cytometers is fairly good. As a rough test of the > isolation > capacity, you can probably feel vibrations on the outside of the > instrument, but > not on the optical bench or the detector housings. > > Dave Coder > dcoder1@qwest.net > > > ----- Original Message ----- > From: Fischer, Randy (NIAMS) <fischer1@mail.nih.gov> > To: cyto-inbox > Sent: Friday, May 25, 2001 4:25 PM > Subject: RE: How much vibration is a problem? > > > > > > Paul, > > > > I completely agree. All one has to do is check out all the various > > locations around our wonderful NIH campus to see that it is not, > for the > > most part, the location that determines whether you get good flow > data. > > Building 10, the clinical center, has so much vibration from all > the > > construction that it is hard to weigh out reagents, but all 3 of > our > > machines are performing beautifully. Now if I could only get that > corner > > lab... > > > > Happy Memorial Day to all, > > > > Randy T. Fischer > > NIH/NIAMS > > Building 10, Room 6D65 > > 9000 Rockville Pike > > Bethesda, MD 20892 > > (301) 594-3537 > > fischer1@mail.nih.gov > > > > > ---------- > > > From: J.Paul Robinson > > > Reply To: jpr@flowcyt.cyto.purdue.edu > > > Sent: Thursday, May 24, 2001 10:07 PM > > > To: Cytometry Mailing List > > > Subject: Re: How much vibration is a problem? > > > > > > > > > Well at the risk of being unscientific and ignorant....I am going > to > > > weigh in on this one... > > > > > > 23 years ago when we bought our first cytometer..we were told > > > "you must have it in the basement....vibration will kill > you....etc etc" > > > ...we put it on the 2nd floor of a 100 year old building, > surrounded > > > by busy roads.....no problems > > > > > > At Michigan when I was there they renovated the entire basement > > > and created a beautiful flow unit, but a dungeon.... > > > > > > Here at Purdue - before I came here, someone decided that the > > > flow had to be in the basement.....we have beautiful lab, no > > > windows, bla, bla...and most of us are starting to look like > > > moles...(well I am anyway) > > > > > > I think 99% of the perception that vibration is a problem is > > > overdone......pick the corner lab, with the best view and go for > it... > > > > > > Paul Robinson > > > (Committee for prevention of cruelty to flow lab people) > > > > > > > > > > > > > > > > > > Date sent: Wed, 23 May 2001 17:12:27 +0100 > > > From: Richard Grenfell <rlg@mrc-lmb.cam.ac.uk> > > > To: cyto-inbox > > > Subject: How much vibration is a problem? > > > > > > > > > Hi everyone > > > > > > I'm new to the list and new to cytometry. > > > > > > We have a Cytomation MoFlo and are considering moving it to > > > another > > > lab (larger hopefully!) > > > > > > The question of vibration has come up. Now, not being used to > > > these > > > instruments, I wondered if anyone here could help. > > > > > > How would I go about measuring vibration in a lab and how much is > > > 'too > > > much'. > > > > > > Also, as a general rule of thumb, is it best siting these > instruments > > > as low down in a building as possible, though fairly near a road, > or > > > higher up in the building? > > > > > > Any help would be appreciated. > > > > > > Thanks > > > > > > Richard Grenfell > > > > > > -- > > > Richard Grenfell > > > Laboratory of Molecular Biology > > > Medical Research Council > > > Cambridge > > > rlg@mrc-lmb.cam.ac.uk > > > J.Paul Robinson, PhD PH:(765)4940757 > > > Professor of Immunopharmacology > > > Professor of Biomedical Engineering > > > Purdue University FAX:(765)4940517 > > > EMAIL:jpr@flowcyt.cyto.purdue.edu > > > WEB: http://www.cyto.purdue.edu > > > > > > > > > __________________________________________________ Do You Yahoo!? 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