Hi everybody, I was trying to do FACS analysis on stem cells transplanted SC into mice. after few days the cells form cartilagenous/bone tissue. I tried to recover the cells by treatment with 0.2% collagenase in PBSx1 for 1 hr. most of the recovered cells were dead cells. Does anybody have recommendations how to do this with keeping the maximal cell viabilty?? any comments will be appreciated, regards, Hadi +-------------------------------------------------- Hadi Aslan MSc./D.M.D. student Laboratoy of Molecular Pathology-Prof. Dan Gazit School of Dentistry-Hadassa Ein-karem Hebrew University of Jerusalem-Israel Cellular : 051-450259 Lab Tel: 972-2-6757625 +--------------------------------------------------
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