Sulforhodamine 101 is a red-fluorescent alternative to FITC for total protein
staining in flow cytometry. Its fluorescence is compatible with all the GFP
mutants.
Biotech Histochem 1997 Jan;72(1):1-9
Flow cytometric applications of Sulforhodamine 101 as a
fluorescent stain for total cellular protein.
Engelhard HH.
Department of Surgery, Northwestern University Medical
School, Chicago, Illinois 60611, USA.
In this paper, the use of Sulforhodamine 101 (SR 101;
C.I. 14318) as a fluorescent stain for flow cytometric determinations of total
cellular protein (TCP) is described. Flow cytometric
quantification of TCP fluorescence can provide a valuable analytical parameter
for
assessing both changes occurring in overall cellular
protein content, such as in response to blast transformation, and heterogeneity
in
cellular size within a specimen, such as a tumor. Very
little information is available in the literature pertaining to the use of SR
101 as a
protein stain. Like fluorescein isothiocyanate (FITC),
SR 101 can be excited at 488 nm; however, it binds ionically and has an
emission maximum at 600 nm, which is advantageous in
certain staining and filter combinations. In this report, the utility of SR 101
staining is demonstrated using pokeweed
mitogen-stimulated lymphocytes and cycloheximide- and dimethylsufloxide-treated
cells.
Single, two- and three-color flow cytometric
applications are possible, using SR 101 in combination with
4',6-diamidino-2-phenylindole (DAPI) and/or FITC.
Elena Soriano wrote:
> I please for an advice about what fluorochromes could I use to stain RNA in
> presence of GFP. For RNA I allways used pyronyne-Y, but in this case its
> fluorescence is strongly overlapped by GFP. I have the same trouble with
> FITC for total protein content.
>
> Does someone knows what alternatives could I have and where could I
> purchase them?
>
> Thank you.
>
> Elena.
>
> -----------------------------------------
> Elena Soriano
> c/o Institut für Angewandte Mikrobiologie
> Univ.f.Bodenkultur Wien
>
> Tel: +43 1 36006 6241
> Fax: +43 1 36 97 615
> http://www.boku.ac.at/iam
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