Hi, I was wondering if anyone could help me. I am trying to synchronize amniotic fluid cells in G1, S and G2 phases of the cell cycle. I have never done this before and I do not have a working protocol to start with. Till now I've tried the serum withdrawal for 48 h for G0/G1, and for S phase - 48h of serum starvation followed by 15 h of incubation with aphidicolin (6uM); serum withdrawal allowed me to get 85% of cells in G0/G1, the method for S phase synchronization I used yielded in 75% of cells at S phase. I would really appreciate if someone could provide me with a protocol that would give a better efficiency of synchronization with regards to amniotic fluid cells. Thank you in advance, Wojciech Niedzwiedz University of Bristol AOC Orthopaedic Center __________________________________________________ Do You Yahoo!? Yahoo! Auctions - buy the things you want at great prices http://auctions.yahoo.com/
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