Dear all, I've recently analysed and sorted a population of bacteria that were stained with a fluorescent antibody. Using the fluorescent cells I set the FSC to log amplification and used SSC (linear) as the thresholding parameter. This produced great results. However I was a little dubious of using the ssc (which was weak and ill-defined) as the threshold as it was difficult to distinguish were "noise" finished and the cell population actually began. Any tips or suggestions? Many thanks, Jeff Barry Flow Cytometry Paterson Institute Manchester UK
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