Hello--I am an extreme novice in the field of flow, and I have a question about an existing protocol that I am attempting to replicate. I am trying to label a nuclear protein (PARP). The authors of the protocol resuspended the fixed and permeabilized cell pellets with 1% BSA in PBS followed by incubation with the specific FITC-conjugated Ab. Following the incubation, the cells were pelleted again and washed with 1% BSA-PBS to remove excess stain. Here's my question; what is the purpose of the BSA in PBS prior to and following incubation of the Ab? Also, there are a vast variety of BSA available; what would be the best in this application? Any information would be appreciated, Matt. ============================ Matthew B. Schabath Predoctoral Fellow Department of Epidemiology M.D. Anderson Cancer Center lab: 713-792-5760 office: 713-745-1078 fax: 713-792-0807
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