I need to stimulate human peripheral blood lymphocytes into proliferation, with the special requirement that all types of lymphocytes be maximally stimulated. With the shortest possible lag-time. And good long-term growth if I keep diluting them. Can anybody give me a state of the art protocol, or point me to useful publications, or commercial media that are formulated for this purpose ? Many thanks in advance for your help Ralph M. Bohmer, Tufts Medical School, Boston
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