Re: Primary Human cells

From: Simon Monard (smonard@trudeauinstitute.org)
Date: Tue May 15 2001 - 08:59:42 EST


I would not  sort primary human or primate material without some sort of aerosol
containment, wearing a mask for more than an hour or two is very uncomfortable. I have
a custom Class one  type hood on my Vanatge



Simon Monard
FACS Lab Manager
Trudeau Institute
Saranac Lake
NY12983

Ph 518 891 3080 X352


>>> Bill Nostrom <bnostrom@caregroup.harvard.edu> - 5/11/2001 2:28 PM >>>

Hi All

What are your thoughts about the safety in sorting primary human cells?
I am concerned that with the sorters, aerosols are produced and anything
biohazard can fly about the room. My users "say" that they have all been
tested, but they also get samples from all over the world.  An
additional factor is that my sorter is in the same room as my FACScan,
so am I potentially risking others to a biohazardous environment? Should
I make it policy that anyone who works in the lab wear a mask? Your
thoughts are appreciated.

Regards ,
Bill Nostrom
Lab Manager
Beth Israel Deaconess Medical Center
Boston, Mass



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