Jian, I wish I had as nice results as yours. Caspace-3 indicates very early internal apoptotic changes which preceeds PS translocation to the external membrane of the cells. It is the exposed PS that annexin V detects. John Waitumbi ----- Original Message ----- From: "Jian She" <Shej@war.wyeth.com> To: cyto-inbox Sent: Tuesday, May 08, 2001 11:14 PM Subject: Re: About double positive population of Annexin V andCaspase-3 Dear Dr. Darzynkievicz or other flow experts: In my experiment for Apoptosis, I have a difficulty to understand population (Caspase-3 "+" and Annexin V "-" cells. Could you help me to explain this population?!! Attached is the FACS analysis results. Thanks; Jian She >>> <mgumanovskaya@pharmingen.com> 05/08 2:09 PM >>> Dear Dr. She, Thank you for your interest in PharMingen's and Transduction Laboratories' reagents. I am having a hard time to explain why are you getting Caspase-3 "+" and Annexin "-" cells . I am going to pick other peoples brain :) , but I would also recommend for you to send an e-mail to flow cytomerty mailing group. It is great way to people like Dr. Darzynkievicz and other to help with your question. You can address it: Dear flow experts :) or something like that. Many flow users read this every day and chances are somebody had same issue. Email address is : cyto-request@flowcyt.cyto.purdue.edu Best regards, Dr. Marina Gumanovskaya Technical Service BD Biosciences PharMingen and Transduction Laboratories "Jian She" <Shej@war.wyeth.com> on 05/02/2001 02:43:39 PM To: cyto-inbox cc: Subject: About double positive population of Annexin V and Caspase-3 Please focus on: The phenotype of Annexin V negative and Caspase-3 positive population. What is biological mean? Jian She, M.S, M.D. Senior Research Scientist I Wyeth-Lederle Vaccines Bldg. 190/Rm. 4118 401 N. Middletown Rd. Pearl River, NY 10965 Phone: 845-732-4836 Fax: 845-732-3628 SHEJ@war.wyeth.com
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