So, what would you recommend for the LSR? ---------------------- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on 05/10/2001 10:26 --------------------------- J.Webster@centenary.usyd.edu.au on 09-May-2001 18:21 To: cyto-inbox cc: Subject: Re: Power requirements for Alexa350 excitation At 21:47 9/5/2001, David_C_McFarland@sbphrd.com wrote: >Wayne, >What wavelength does your UV laser operate at? I'm about to try Alexa 350 >on an LSR which has a 325nm UV laser and it has been intimated to me that >it may not work very well. And I won't have the option of adjusting >output. I for one would be interested in your results. >David McFarland We did try Alexa 350 on an LSR, the best I can say is "disappointing..." There was a detectable signal, but not enough separation to do anything useful; if I remember correctly we used something like CD4. I plan to try it on the sorter next time I run an argon laser in multi-line UV, that may not be soon though. Joseph. -- Joseph Webster, Flow Cytometry Facility Centenary Institute, Sydney AUSTRALIA.
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