I have not found large bead sets comparable to the Luminex set. BD does not seem interested in distributing the beads that go into their Cytometric Bead Array in a form that users can modify. Molecular Probes sells a couple of colors and sizes of streptavidin beads but not a large set that can be used easily together, and is apparently not planning to expand the set. The best source I've found is Bangs Laboratories (www.bangslabs.com), which sells a few types of beads that can be used for setting up multiplex assays in a similar fashion to the BD array. The beads fluoresce at 5 intensities in the FL3 channel and are available in at least 2 sizes, 4.4 and 5.5 um. The beads carry either goat-anti-mouse IgG or streptavidin, and beads carrying only COOH groups will be available soon. I tried a set and saw nice separation in FL3 (FACSCalibur) of 4 dyed bead peaks and 1 unstained bead set, with a low-intensity (autofluorescence?) peak in FL2 and no signal in FL1, so they should be suitable for use with FITC-like secondary labels. Other sizes might be possible to expand this set. Bangs Laboratories seems to be in the process of developing these further, so it's worth contacting them. Laird Bloom Phylos, Inc. 128 Spring Street Lexington, MA 02421 Tel. (781) 862-6400 ext. 253 e-mail: lbloom@phylos.com > ---------- > From: Fang-Yao Stephen Hou > Sent: Saturday, May 5, 2001 10:07 PM > To: Cytometry Mailing List > Subject: Luminex64 multiplex beads > > > Does any know where to purchase the Luminex64 beads for multiplex > analysis by flow cytometry? The LuminexCorp discontinued this product > and promote the stand alone instrument, Luminex 100, which costs about > US$ 45000. In addition, the new multiplex beads can not be used > on the old instrument, according to the people there. It becomes too > expensive to try this versatile multiplex technology. Or are there other > manufactures with similar products? Thanks. > > Stephen Hou > UC-Irvine > _________________________________________________________________ > Get your FREE download of MSN Explorer at http://explorer.msn.com >
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