Just a very quick question - can someone with experience of Alex350 use in flow tell me how much laser power is usually needed to get a decent signal? I have a neutravidin Alexa350 that stubbornly refuses to show itself and I can't go over 50 mW or else I'll start leaving burn marks on my UV line LP splitter. Cheers Wayne W Turnbull Flow Cytometry Services Immunobiology Guys Hospital London
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