FYI Calman, Abé Schwartz had such a kit available for *intracellular* quantification of cytokines, too. It was called the "Quantum Simply Cellular Kit" and was sold by our company around '97. I don't know if any of our customers compared the results to an ELISA. A reference for this kit (no comparison to ELISA included) Agrawa et al. Cytometry 17: 237-245 (1994) Hope this helps some. Hans Beiderbeck, PhD Hoelzel Diagnostika GmbH www.hoelzel.de ----- Original Message ----- From: Calman Prussin To: Cytometry Mailing List Sent: Monday, April 30, 2001 4:49 PM Subject: RE: validation of intracellular staining with beads A follow-up to this. I did the experiment looking at intracellular tryptase and got a correlation r=0.48 when I compared the MESF to that for ELISA. > ---------- > From: Calman Prussin > Sent: Wednesday, February,21, 2001 20:01 > To: 'Cytometry Mailing List' > Subject: validation of intracellular staining with beads > > Does anyone have a reference that has compared intracellular staining > quantitation using beads and mean equivalent soluble flurochrome (MESF) > with immunologically detected intracellular protein by ELISA? These types > of validation studies have been done by Abe Schwartz and others for > EXTRACELLULAR antigens, but I could not find such a citation for > INTRACELLULAR antigens. Thanks. > > Note to would be do-gooders: I am NOT looking for validation of > intracellular cytokine staining by studying secreted cytokine NOR am I > looking to validate the percent positive staining. I need to validate > intracellular protein MESF. > > Surfs up, gotta' go, > > Calman > _______________________ > Calman Prussin > Laboratory of Allergic Diseases > NIAID/ National Institutes of Health > > > >
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