RBC lysis on ice

From: Pia Kivisakk, Ph.D. (kivisap@ccf.org)
Date: Thu May 03 2001 - 12:04:18 EST


Dear Flowers,

I need to perform a lysed whole blood staining (human) strictly on ice, but the lysing
solution that I presently use (from BD) doesn't work well at this low temperature. Are
there any other lysing solutions that can be used on ice?

Many thanks!

Pia Kivisäkk (kivisap@ccf.org)
Reseatch Fellow
Lerner Research Institute
Cleveland Clinic Foundation



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