Sam Witherspoon wrote- > For years I have been adjusting the beam *focus* on our sorter with >help from a pulse width signal, in this case, FSC width. The idea is that >the smallest width represents the smallest beam spot. > *I use glut.-fixed chicken red blood cells on a FACStar Plus. (100u nozzle) >with an elliptical spot. > *488nM laser at 60mW. > *The CV's I get on PI-stained CTN are 3% at 256 channel resolution, which I >believe for this type of instrument is quite good. As long as the shortest pulse width is associated with the biggest signal and the lowest CV, you're OK. The potential problem is moving the beam so the core is near the edge rather than the center of the ellipse, which could potentially give you shorter pulses, which would be of relatively low amplitude and high CV. I hope to have some of the hands on Cytomutt stuff which was axed from the 3rd Edition on the Web site which will be set up for the 4th Edition. -Howard
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