Tony Schountz wrote: -----Original Message----- From: Tony Schountz [mailto:tschount@mesastate.edu] Sent: Wednesday, April 25, 2001 3:27 PM To: Cytometry Mailing List Subject: Making monoclonals to cell surface molecules I'd like to solicit opinions on producing monoclonal antibodies to cell surface proteins. I will be immunizing BALB/c mice with thymocytes or splenocytes from a deer mouse, a species that is much more divergent from lab mice than rats are. I will be screening hybridoma supes by flow cytometry so that any antibody I detect will be to cell surface proteins only. One question that I have is whether it is necessary to emulsify the deer mouse cells in CFA/IFA for immunization/boost. Most papers are unclear on this, while some specifically say that this was done. Goding's book states that this is unecessary, while Current Protocols in Immunology doesn't really address whole-cell immunizations. Any tips or suggestions will be greatly appreciated. Thanks, Tony -- Tony Schountz, Ph.D. Department of Biological Sciences Mesa State College mailto:tschount@mesastate.edu You might want to consider Hunter's TiterMax adjuvant. It is not as toxic as CFA and IFA, and I have gotten excellent results in BALB/c that were immunized with protein. There number is 800-345-2987. Good Luck Delynn Moss Centers for Disease Control
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