Julie, others . . . If you look at the web site, you'll see reference to Mouse, Rat, and Human reagents. Nowhere does it say that the data shown is for Human only . . . a tad misleading, no? MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Flow Core 7416 CCGC 0946 (734) 647-3216, fax (734) 936-7376 kukuru@umich.edu >>> Julie Auger <jauger@flowcity.bsd.uchicago.edu> 04/24/01 05:43PM >>> Mark - It seems this link only advertises anti-human antibodies. It has been seen by many labs, including mine, that PE-Cy5 conjugates bind to B cells from the NOD mouse strain, which Keith is referring to. Julie P.S. I also do not believe this is an Fc receptor binding, as we routinely use an antibody against Fcgamma II/III Receptor (clone 2.4G2) when we stain. At 09:23 AM 4/24/01, you wrote: >Keith, >So, are you disputing Caltag's claim? (see < www.caltag.com > ; "New Tri-Color >Conjugates" link). >MAK. > > >-- >Mark A. KuKuruga, Managing Director >University of Michigan Flow Core >7416 CCGC 0946 >(734) 647-3216, fax (734) 936-7376 >kukuru@umich.edu > > > >>> Keith Bahjat <kbahjat@ufl.edu> 04/21/01 02:59PM >>> > >Joe and others, > >As far as the binding of Cy5 to NOD B cells goes, I can tell you the new >formulations of PE-Cy5 DO NOT correct this problem . . .
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