PI and fixation

From: Da Silva, Marcos (Marcos.DaSilva@umassmed.edu)
Date: Wed Apr 25 2001 - 15:47:29 EST

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Maybe it is worth reminding that cells treated with BD FACSLYSE are fixed,
even if FACSLYSE is washed out, and will became PI-positive. When comparing
samples treated with FACSLYSE with samples treated with a non-fixative
Ammonium Chloride solution, I observed that the percentage of  PI or 7-AAD
positive cells is much higher in the first group.

Marcos da Silva
Pediatric Immunology lab
UMassMed - Worcester

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