I agree with Simon that Accudrop is the easiest way to set up drop delay. It sure beats sorting on to slides and counting beads. Best of all you can check drop delay before every sort (as if you want to sort onto slides and count beads several times a day!). I am very confident that I can accurately set drop delay for one droplet envelope or any other drop envelope. I also agree with Simon that having the laser intersecting stream and sort streams allows for the ability to monitor streams during sort. David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CNY rm7017 149 13th Street Massachusetts General Hospital-East Boston, MA 02129 Tel. (617)726-1683 Fax.(617)724-3164
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