I apologize for my earlier impolite reply concerning PI. I am extremely busy and just firing off answers without review for how they sound. I just wanted to let the person asking know- PI is not great for viability after fixation. I just must not answer when I am so pushed for time. I hate it when I realize I've been insensitive and rude- which I was. Maryalice Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH "Learn the rules so you know how to break them properly." The Dalai Lama
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