Abby, One suggestion . . . do a lit. search. For example, see the following papers dealing with octeoclasts and flow: 1. Sandhu JS. Gorczynski RM. Waddell J. Nguyen H. Squires J. Waddell J. Boynton EL. Hozumi N. Effect of interleukin-6 secreted by engineered human stromal cells on osteoclasts in human bone. Bone. 24(3):217-27, 1999 Mar. 2. Dougall WC. Glaccum M. Charrier K. Rohrbach K. Brasel K. De Smedt T. Daro E. Smith J. Tometsko ME. Maliszewski CR. Armstrong A. Shen V. Bain S. Cosman D. Anderson D. Morrissey PJ. Peschon JJ. Schuh J. RANK is essential for osteoclast and lymph node development. Genes & Development. 13(18):2412-24, 1999 Sep 15. 3. Sakai A. Nishida S. Okimoto N. Okazaki Y. Hirano T. Norimura T. Suda T. Nakamura T. Bone marrow cell development and trabecular bone dynamics after ovariectomy in ddy mice. Bone. 23(5):443-51, 1998 Nov. 4. Tsurukai T. Takahashi N. Jimi E. Nakamura I. Udagawa N. Nogimori K. Tamura M. Suda T. Isolation and characterization of osteoclast precursors that differentiate into osteoclasts on calvarial cells within a short period of time. Journal of Cellular Physiology. 177(1):26-35, 1998 Oct. 5. Kelly KA. Tanaka S. Baron R. Gimble JM. Murine bone marrow stromally derived BMS2 adipocytes support differentiation and function of osteoclast-like cells in vitro. Endocrinology. 139(4):2092-101, 1998 Apr. 98187847 Abstract | Complete Reference 6. Miyamoto A. Kunisada T. Hemmi H. Yamane T. Yasuda H. Miyake K. Yamazaki H. Hayashi SI. Establishment and characterization of an immortal macrophage-like cell line inducible to differentiate to osteoclasts. Biochemical & Biophysical Research Communications. 242(3):703-9, 1998 Jan 26. 7. Yao GQ. Sun Bh. Hammond EE. Spencer EN. Horowitz MC. Insogna KL. Weir EC. The cell-surface form of colony-stimulating factor-1 is regulated by osteotropic agents and supports formation of multinucleated osteoclast-like cells. Journal of Biological Chemistry. 273(7):4119-28, 1998 Feb 13. 8. Iotsova V. Caamano J. Loy J. Yang Y. Lewin A. Bravo R. Osteopetrosis in mice lacking NF-kappaB1 and NF-kappaB2 [see comments]. Nature Medicine. 3(11):1285-9, 1997 Nov. 9. van Beek ER. Lowik CW. Papapoulos SE. Effect of alendronate treatment on the osteoclastogenic potential of bone marrow cells in mice. Bone. 20(4):335-40, 1997 Apr. 10. Hayashi S. Miyamoto A. Yamane T. Kataoka H. Ogawa M. Sugawara S. Nishikawa S. Nishikawa S. Sudo T. Yamazaki H. Kunisada T. Osteoclast precursors in bone marrow and peritoneal cavity. Journal of Cellular Physiology. 170(3):241-7, 1997 Mar. 11. James IE. Dodds RA. Lee-Rykaczewski E. Eichman CF. Connor JR. Hart TK. Maleeff BE. Lackman RD. Gowen M. Purification and characterization of fully functional human osteoclast precursors. Journal of Bone & Mineral Research. 11(11):1608-18, 1996 Nov. 12. Bowler WB. Birch MA. Gallagher JA. Bilbe G. Identification and cloning of human P2U purinoceptor present in osteoclastoma, bone, and osteoblasts. Journal of Bone & Mineral Research. 10(7):1137-45, 1995 Jul. 13. Masi L. Brandi ML. Robey PG. Crescioli C. Calvo JC. Bernabei P. Kerr JM. Yanagishita M. Biosynthesis of bone sialoprotein by a human osteoclast-like cell line (FLG 29.1). Journal of Bone & Mineral Research. 10(2):187-96, 1995 Feb. 14. Chuntharapai A. Bodary S. Horton M. Kim KJ. Blocking monoclonal antibodies to alpha V beta 3 integrin: a unique epitope of alpha V beta 3 integrin is present on human osteoclasts. Experimental Cell Research. 205(2):345-52, 1993 Apr. 15. Popoff SN. Jackson ME. Koevary SB. Marks SC Jr. Coexistence of reduced function of natural killer cells and osteoclasts in two distinct osteopetrotic mutations in the rat. Journal of Bone & Mineral Research. 6(3):263-71, 1991 Mar. 16. Wioland M. [Electrophoresis applied to the analysis and separation of cells]. [French] Biomedicine & Pharmacotherapy. 40(2):64-74, 1986. 17. Schneider GB. Cellular specificity of the cure for osteopetrosis: isolation of and treatment with pluripotent hemopoietic stem cells. Bone. 6(4):241-7, 1985. 18. Bar-Shavit Z. Teitelbaum SL. Reitsma P. Hall A. Pegg LE. Trial J. Kahn AJ. Induction of monocytic differentiation and bone resorption by 1,25-dihydroxyvitamin D3. Proceedings of the National Academy of Sciences of the United States of America. 80(19):5907-11, 1983 Oct. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Flow Core 7416 CCGC 0946 (734) 647-3216, fax (734) 936-7376 kukuru@umich.edu >>> "Kelliher, Abigail S." <ASALLEN@PARTNERS.ORG> 04/17/01 02:15PM >>> Hi I have a researcher who is interested in identifying circulating osteoclasts by flow. I looked in the archives where this question has been posted before, but I saw no results. Has anyone any suggestions? Abby Kelliher Clinical Flow Cytometry Laboratory Mass. General Hospital Boston, MA 02114
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