CD34 enumeration

From: Piechota, Tracie E (Tracie_Piechota@dfci.harvard.edu)
Date: Wed Apr 18 2001 - 10:28:37 EST

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Hi.
	 My question deals with CD34 enumeration. I was wondering if anyone
has experience working with the Beckman-Coulter StemKit or the B-D ProCount
Kit. Our lab currently uses the StemKit and we have been having a lot of
trouble getting the stemtrol procedural control to be within 10-15% of the
assayed value on a daily basis. We believe our technique is correct, but the
value for the control continues to fluctuate. We have considered switching
to the ProCount Kit, but I understand it has limited applications. Our lab
processes PBMNCs, stem cells, cord blood, and bone marrow. Any help would be
appreciated. Thanks.


Sincerely,

Tracie Piechota

Cell Processing Tech II

email: tracie_piechota@dfci.harvard.edu

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