Marty, Interesting question. I have always instructed people that bring me cells to do so on a consistent basis with respect to when they harvest the cells. Same number of days in culture, same number of splits post sort, I strive to have every variable approach a constant. To simply make things easier to troubleshoot. Typically, they are working with confluent growth at the time we sort them. If for no other reason than to simply have enough cells. I can't provide any insight as to whether waiting for confluency enhances or harms the survival post sort. I will look forward to more on this topic. chris MoFlo Pilot On Sun, 1 Apr 2001 11:47:18 -0700, Marty Bigos wrote: > >Hi- > >A grad student here asked me the following question. I couldn't come >up with any hard evidence from my experience so I would like to ask >for any feedback that others may have. Thanks. > >Marty Bigos >Gladstone Flow Core > >"I was wondering what you knew about the affect of sorting a cell >line on the health of the cells. Is it thought that you get >healthier cells out post-sort if the cells start from a confluent or >not yet confluent plate? Does it matter?" >
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