I have just been working with a student who is wanting to count bacteria from estuarine and coastal samples from oyster feeding experiments. We use SYBR Green II to count bacterial samples from the open ocean and have had few problems with separating the population from background. With the estuarine and coastal samples with SYBRII the background noise is elevated and differentiating the population becomes difficult. Any body used a more effective stain or found a better way of approaching this??? Julie ---------------------------------- Julie Hall Manager Aquatic Ecology and Laboratory Services NIWA, Box 11-115, Hamilton, NZ Ph. +64-7-856 1709 Fax +64-7-856 0151 eMail j.hall@niwa.cri.nz
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