The mouse monoclonal antibody CH-11 had been reported to recognize rat Fas. See Watson et al. Surgery, 1997; 122: 163-172 and Muschen et al. Gasteroenterology 1999; 116: 666-677 for details. This antibody was originally described to be specific for human Fas and is sold by a number of companies including Kamiya. Since CH-11 works with rat Fas, I will not be surprised that human FasL may also bind to rat Fas. If CH-11 works with rat Fas, I would try IPO-4 since it is also an IgM antibody but is more potent in killing at least human T cells in my hands. Let me know about your luck with rat cells with these antibodies. Good luck! Sundararajan Jayaraman, Ph.D. Research Assistant Professor Dept. of Pathology University of Miami Medical School DRI Building Room 5018 (R-134) 1450 NW 10th Avenue Miami, FL 33136 > -----Original Message----- > From: Sraboni Ghose [SMTP:sraboni@stanford.edu] > Sent: Wednesday, April 04, 2001 8:44 PM > To: Cytometry Mailing List > Subject: rat anti-Fas antibody? > > > Hello, > I want to induce apoptosis in primary cultures of rat hippocampal neurons > using the > Fas signalling pathway. I have searched through many catalogs for an anti > Fas > receptor antibody that would trigger the Fas response (similar to the Jo-2 > from > Pharmingen for mouse cells/lines) but I haven't found any. Can anyone > direct me to a > supplier please? Does anyone know of a source of rat Fas ligand that I > could use > instead? > Thank you for your help > Sraboni
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