The best for this would be to ficoll the blood then store the mononuclear cells at 4 degrees. They should keep well for several days (stained or unstained). whole blood that is going to be stored for more than 24 hrs should be collected in ACD. Let this set at room temp until needed, preferably with the tube on its side. Large amounts of blood can be transfered to a Life Cell bag and left at RT. Jim Houston -----Original Message----- From: Jan Hesketh [mailto:j.hesketh@auckland.ac.nz] Sent: Tuesday, March 27, 2001 4:51 PM To: cyto-inbox Subject: Time delay before sorting Could anyone please tell me if there are any problems associated with a 2 day delay between blood collection and processing for T cell sorting. I have a bone marrow transplant patient and we are sorting T cells for further analysis (not culture). Normally the blood is separated on a ficoll gradient then the cells stained with a CD3 marker and sorted. Time restraints may mean that I cannot process the blood immediately. If a delay is possible, what would be the best conditions for storage of the whole blood or separated cells? Thanks in anticipation, Jan Hesketh Flow Cytometry and Cell Sorting Facility Molecular Medicine University of Auckland New Zealand
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