Dear Tomas, there goes my neck: I've ran a similar kit from OrpeGen Pharma. I have processed maybe 1-2000 samples in the last several months for neutrophil and monocyte oxidative burst. we use both the percent shift and geometric mean change. for example: monocytes control =1%, high control=98%, we use 97%. neutrophil GM control = 120, monocyte GM high control = 3500 , we use the difference. Although not contained within the instructions, we manually compensate the green rhodamine signal vs. the PI signal (yes, they overlap, and yes, the data looks different without compensation). Also if you're using GM, you should make sure your starting GM is in the same neighborhood for each new sample... voltage may need to be changed. There are patients who come in febrile, septic or otherwise insulted and they may get an extra "peak" in the unstimulated control, which is normal, but has to be accounted for in the analysis - this may increase the % substraction in your %-age calculations. regards, Maciej __________________________________________________ Do You Yahoo!? Get email at your own domain with Yahoo! Mail. http://personal.mail.yahoo.com/?.refer=text
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