To the ongoing discussion on the interpretation of the Annexin V- PI binding data let me add one more comment. Annexin V - positive (PI negative) may also be macrophages or other cells that egulf apoptotic bodies (e.g. see Marquet et al., Nature Cell Biol., 1: 454-456, 1999). Apparently, a fusion of plasma membrane of apoptotic bodies with plasma membrane of the engulfig cell makes the latter Annexin V positive. Zbigniew Darzynkiewicz Brander Cancer Research Institute New York Medical College 19 Bradhurst Ave. Hawthorne, NY 10532 tel: 914-347-2801 fax: 914-347-2804 http://www.geocities.com/z_darzynkiewicz
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