Hello again, I am very sorry to have wasted your time giving you wrong information about the problem with the SSC sensitivity. Actually I meant a problem with FL2 sensitivity. I have corrected the question and once again apologise for the mistake. To answer a question that has already been raised, the flow cell is new (about 8 months old) and I try to maintain as clean a system as possible. The modified Q is below. "We have an old FACScan linked to a HP platform. It has been OK until recently. When I caliberate the machine (with Autocomp)FL2 sensitivity fails as the lower limit is not detected. I have tried three lots of Calibrite beads (all within expiry, but all three were not uniform suspensions), but the problem remains. The BD service engineer also tried swapping the FL2 and FL3 PMT's unsuccessfully (is it allowed?). He has assured me that the FL2 sensitivity failing will not affect CD4 counts with Simulset. Any input as to whether the problem is with the PMT or the Calibrite beads is welcome. I would like to be sure that the problem is with the PMT before purchasing it. Thanking you in advance. With warm regards, Sandeep. ===== Dr. Sandeep Ramalingam, Department of Clinical Virology, Christian Medical College Hospital, Vellore-632004, India. Phone(off): 91-416-222102, ext 2070 Fax: 91-416-232035 Email: sandeeprk@yahoo.com __________________________________________________ Do You Yahoo!? Get email at your own domain with Yahoo! Mail. http://personal.mail.yahoo.com/
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