Problem with SSC sensitivity

From: sandeep ramalingam (sandeeprk@yahoo.com)
Date: Wed Mar 21 2001 - 08:28:13 EST


Hello,

We have an old FACScan linked to a HP platform.  It
has been OK until recently.  When I caliberate the
machine (with Autocomp)SSC sensitivity fails as the
lower limit of SSC is not detected.  I have tried
three lots of Calibrite beads (all within expiry, but
all three were not uniform suspensions), but the
problem remains.  The BD service engineer also tried
swapping the FL2 and FL3 PMT's unsuccessfully (is it
allowed?).  He has assured me that the SSC sensitivity
failing will not affect CD4 counts with Simulset.

Any input as to whether the problem is with the PMT or
the Calibrite beads is welcome.  I would like to be
sure that the problem is with the PMT before
purchasing it.

Thanking you in advance.
With warm regards,
Sandeep.

=====
Dr. Sandeep Ramalingam,
Department of Clinical Virology,
Christian Medical College Hospital,
Vellore-632004, India.
Phone(off): 91-416-222102, ext 2070
Fax: 91-416-232035
Email: sandeeprk@yahoo.com

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