You will get much more usable power from the 532 DPSS than from the 543 HeNe. Be careful. "Piping" is the name of the evil transmission of laser light through your fluid stream, to be picked up where you least want it. Just because you "time gate it out" does not protect you (your signals, actually) from scattered light. If you don't plan to use your "green" filter at all (or any filter which overlaps 532 nm), then you shouldn't have a problem. Let us know what you decide, and how it works out! Andrew Beernink Novasite Pharmaceuticals, Inc. 3520 Dunhill St. San Diego, CA 92121 (858) 638-8584 -----Original Message----- From: Arnold Pizzey [mailto:a.pizzey@ucl.ac.uk] Sent: Thursday, March 15, 2001 4:51 AM To: cyto-inbox Subject: Violet and Green Lasers Greetings all, A couple of quick questions.. I see that the Nichia Corporation is producing 5mW 405nM laser diodes (price ~$2000 with an expected MTBF of 15,000 hours) Does anyone know if Hoechst would give a DNA signal if excited at this wavelength?.... I am thinking of adding a green laser to my elite -I would be using it in time gated mode so I'm not worried about crossover into the green channel- the choices for (cheap)lasers seem to be between 532nM and 543.5nm -I guess my question is; would the 532nM laser be good for much or would I have to go for the (more expensive) 543nM unit Best Regards, Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 (0)20-7679-6234 Fax: +44 (0)20-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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