Hi FLOWers
We are trying to stain endothelial cells with a mouse (IgG) anti human CD39 from serotec
( MCA1268). It is not directly conjugated so we are using a second antibody from them
as well rabbit anti mouse IgG (Star9B).
However, we cant get the staining to work.
We have several years experience in staining so we dont think we are doing anything
wrong.
Has anyone else used this antibody, and if so can you provide us with an outline of
your method.
Heather
Heather Medbury (PhD)
Department of Surgery
Westmead Hospital
Westmead 2145
61-2 9845 7677
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All experiments are preliminary
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"In his heart a man plans his course
but the Lord determines his steps"
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