We have been sorting cells stained with Hoechst dye and followed for 5
days. Cells grow after 5 days of Hoechst. We do not know if there is any
mutagenic effects yet.
Joseph Webster
<J.Webster@centenary.us To: Cytometry Mailing List
<cytometry@flowcyt.cyto.purdue.edu>
yd.edu.AU> cc:
Subject: Re: DAPI as a viability
exclusion dye
03/14/2001 05:45 PM
05:34 15/3/2001, Mark Kukuruga wrote:
>Hi all . . .
>Considering the use of DAPI as an exclusion dye, I'm wondering effects of
>about the (albeit brief) UV irradiation on the cells that we sort
>"viably." Should we worry, or is it negligible?
>MAK.
The same question would apply to sorting viable cells stained for DNA
with Hoescht, I've heard of this being done for a long time; anyone
got experience to relate?
After-effects could either result from UV exposure or from the dye
binding direct to DNA; if there are effects, how would you decide
which cause?
Joseph.
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