Re: FW:

From: Larry Arnold (lwarma@med.unc.edu)
Date: Wed Mar 14 2001 - 09:19:36 EST

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Bill, Emma

If you have access to a CompuCyte Laser Scanning Cytometer this might be the best way to go. The LSC can identify mitotic (as well as G1, S, G2, and newly formed daughter cells) using only a single DNA stain e.g. PI. We have a number of users doing this. Other immunofluorescence markers can be combined with the DNA content and the cells can be analyzed in situ growing on slides/cover slips.

Regards,

Larry

11:46 AM 3/12/2001 -0800, you wrote:

Hello,
I am writing this on behalf of a colleague...
Thanks.
-Bill

-----Original Message-----
From: Emma Shtivelman [mailto:shtivelman@cc.ucsf.edu]
Sent: Tuesday, March 06, 2001 11:02 AM
To: hyun@cc.ucsf.edu
Subject:

Hi Bill,

I am trying to find a good detailed protocol for the use of MPM-2 (or
other) mitosis-specific antibody to quantify mitotic cells by flow
cytometry.

Your advice will be appreciated!

Best regards,

Emma
***********************
Emma Shtivelman, Ph.D.
Cancer Research Institute
University of California San Francisco
2340 Sutter Street
San Francisco CA 94143-0128
Phone: (415) 502-1985
FAX: (415) 502-3179
eshtivel@cc.ucsf.edu

Larry W. Arnold, Ph.D.
Res. Assoc. Prof.
Director, Flow Cytometry Facility
Department of Microbiology and Immunology
Lineberger Comprehensive Cancer Center
CB# 7290
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
Phone: 919-966-1530
FAX: 919-962-8103

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