Dear flowers: I am cloning human T cells specific for viral antigens and have slightly confusing result now when I phenotype the clones. They are all CD3+, all CD8+, all CD4-, all HLA-DR+ and now comes the confusing part 10-30% positive for CD56 and 5-10% CD16 positive (the last stimulation with irrradiated feeders and PHA is 12 days ago, the cells are cultured in IL-2 at 1000 U/ml). What are these cells: 1) remaining feeders? 2) my cultures are not clonal? 3) in vitro phenomenon because of culture condition and stimulation? 4) NKT cells? Help with resolving this is appreciated Cheers Jan
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