Dear Group, Hi. I am going to start some experiments with cord blood. Specifically I will be isolating the mononuclear cells from cord blood and use it for expression studies. These will be activated by PMA/A23187. I would be grateful for any suggestions and hints for procedures and pitfalls regarding the use of mononuclear cells from cord blood. Also what are the differences in the lymphocytes isolated form the pheripheral blood and cord blood (more naive cell? etc). Thanks a TON in advance for any suggestions (references) and am in urgent need for feedback. Rana Nagarkatti (SRF) Molecular Immunology Lab Center for Biochemical Technology Mall Road, Delhi 110007, INDIA. ____________________________________________________________________ Get free email and a permanent address at http://www.netaddress.com/?N=1
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