Just to throw my 2 cents in on the matter - we have employed the use of the APC conjugates for anti-human CD8 and anti-human CD56 with APC for staining with both cynomolgus and rhesus macaques. Both work just fine. I would suggest that you look in Pharmingen's catalog at what antibodies cross-react with nonhuman primates, then turn to the human antibody section and pick the fluorophore you want. You will find that the "human" antibodies are substantially cheaper than the "monkey" antibodies, even when they are the same clone and the same fluorophore. So far the only antibody where that does not apply is the CD3 clone which is not sold as a anti-human reagent. -Doug Reed -----Original Message----- From: mandy cromwell [mailto:mandy_cromwell@hms.harvard.edu] Sent: Wednesday, March 07, 2001 6:05 PM To: cyto-inbox Subject: cross-reactive Ab for NHP flow Peter, If you have a B-D Pharmingen catalog you may have noticed that none of the Abs listed in the NHP section appear to be available as APC conjugates. If you look up the same clones in the human section, however, you will find that several clones are listed as APC conjugates. For example, the CD2 clone RPA-2.10, CD8 clone RPA-T8, CD20 clone 2H7, and CD69 clone FN50 all x-r with rhesus and are listed in the human section conjugated to APC. Also, the CD4 clone SK3 (rhesus x-r) has been listed in the (old) B-D Biosciences immunocytometry catalog as an APC conjugate (though for some reason it isn't in the current B-D Pharmingen). I don't know of any x-r CD28 or L-selectin clones that are available in APC-maybe someone else can help out there. Take a look at Keith Reiman's NHP reagent database for more crossreactivity information: http://rncc.bidmc.harvard.edu/labs/viral/Frameset_CD.html Best regards, Mandy ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~ Mandy Cromwell, PhD New England Regional Primate Research Center Harvard Medical School southborough, MA 01772 (508)624-8022 >I am currently setting up assays for measuring CD antigens on rhesus monkey >T-cells by 4-color flow cytometry. I am trying to identify a source of human >Mabs (e.g. CD2, CD3, CD4, CD8, CD20, CD28, CD62L, CD69, etc) which are >linked to APC and cross-react with rhesus T-cells. > >Your help would be greatly appreciated. > >Peter Silvera, PhD. >Retrovirology Department >Southern Research Institute >431 Aviation Way >Frederick, MD 21701 >Tel: 301-694-3232 >Fax: 301-694-7223 >e-mail: silvera@sri.org > >AND > >Henry Jackson Foundation >HIV Research Program >Rockville, MD 20850. Mandy
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