The brochures/applications for the Dartmouth Flow Course (July 15th - July 20th) are now available. Contact karen.griswold@dartmouth.edu and she will send you one. If you are on the ISAC mailing list, you will get one automatically. In order to have more time for lectures on specific advanced topics during the Course itself, we are giving an optional tutorial session on introductory flow theory on Saturday afternoon, July 14th. This session will include informal lectures and discussion on how a flow cytometer works, choice of fluorochromes, compensation for spectral crossover, staining dos and don'ts, and basic methods for data analysis. There will be plenty of time for questions. The Course itself starts on Sunday, July 15th, with a day of lectures, a banquet, and a keynote address by Zbigniew Darzynkiewicz. The laboratory modules for the 2001 Course are listed below. In addition to the common clinical applications, we will have several "clinical frontier" labs with Nadège Bercovici teaching about tetramer staining, Kathy Muirhead teaching about tracking cells for cell division, Frank Mandy including multiplex bead assays in his HIV lab, and Doug Taatjes running a module on the laser scanning cytometer. 1) Carleton and Sigrid Stewart: multicolor leukemia and lymphoma phenotyping 2a) Kathy Muirhead: cell tracking 2b) Nadège Bercovici: tetramer staining 3a) Mike Keeney: CD34 stem cells 3b) Doug Taatjes: laser scanning cytometry 4a) Kenneth Ault: platelets 4b) Bruce Davis: fetal cells in the maternal circulation 5a) Bruce Bagwell: data analysis for DNA/cell cycles 5b) Paul Wallace, Alice Givan: staining cells for DNA content 6) Jake Jacobberger: multiparameter staining and analysis for intracellular proteins, surface proteins, DNA 7) Frank Mandy and Michele Bergeron: HIV/AIDS, including CD4/CD8 quality control and multiplex bead assays for soluble analytes. Let me know if I can give you any further information (it is rumored that Howard Shapiro and guitar will make a surprise appearance at the lobster bake). Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, New Hampshire NH 03756 tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu
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