>I am just starting to use flow cytometry and am facing some problems: > >1. I am trying to measure intracellular antigen with FITC-labeled Antibody, DNA content with PI and viability of cells stained with EMA prior to fixation at the same time. Has anybody experience with such an approach? Will it work out? >2. What kind of fixation ist best to use for measuring DNA-content and intracellular antigens simultaneously? >3. I am also trying to establish an assay with FITC and PE-labeled Antibodies used parallel to PI-staining of dead cells. I found controversial statements concerning the parallel use of PE-labelling and PI in literature. Has anyone advice? > >thanks very much ;-)) Kerstin Buettner Institute of Biophysics and Radiobiology University of Hamburg Martinistr. 52 20246 Hamburg Phone: 0049-40-42803-2938 Fax: 0049-40-42803-5139 E-Mail: k.buettner@uke.uni-hamburg.de
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