Ca++ of pancreatic cells

From: Len Brown (lbrown@mmri.mater.org.au)
Date: Mon Feb 19 2001 - 18:11:03 EST

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Dear Flow-ers

I have a researcher about to start a project and is seeking advice. Does
anyone have experience of measuring Ca++ flux on rat pancreatic beta cells
(INS-1) using Fluo 3 and Fura red? Any hints on dye loading, positive
controls etc.

Thanks

Len Brown
Flow Cytometry
Mater Medical Research Institute
Brisbane, Australia

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