Actually, hairy cell variant was originally described as the prolymphocytic variant of HCL and required the presence of a prominant single nucleolus. I think it is a little controversial whether the original disease is a distinct entity and I would not confuse it more by lumping other cases with morphologic features atypical for hairy cell into this category. Dan Daniel A. Arber, M.D. Director, Hematopathology and Molecular Pathology City of Hope National Medical Center 1500 East Duarte Road Duarte, CA 91010 (626) 359-8111 x 2456 FAX (626) 301-8145 > -----Original Message----- > From: "Maryalice Stetler-Stevenson" <stetler@box-s.nih.gov>@COHNOTES > Sent: Thursday, February 15, 2001 7:26 AM > To: Cytometry Mailing List > Subject: RE: HCL variant vs. ? > > <<...OLE_Obj...>> > The following, in my book, qualifies this as hairy cell variant: > 1. 1. Morphology is atypical > (TRAP is positive in these cells but morphologically they are not > quite > classical hairy cells.) > 2. They are CD25 negative (CD25 is + in 97% of typical HCL and + in > 0% of vHCL > 3. There is weak to moderate staining for FMC7- 91% + in HCL and 73%+ in > vHCL > 4. CD10 is + in 5% of typical HCL and in 21% of vHCL > The most important point is that it doesn't look like typical HCL. > Maryalice > With the exception of not staining for CD25, everything else fits > for > typical hairy cell leukemia (older gentleman, dry tap on marrow, > pancytopenia). Although CD10 is not common, it has been > well-described in > otherwise typical hairy cell. What qualifies this as a so-called > hairy cell > variant? > Christopher S. Bee, M.D. > Medical Director, Cellular Immunology and Flow Cytometry > Wilford Hall Medical Center > Lackland AFB, Texas > (210) 292-5455 > DSN 554-5455 > christopher.bee@59mdw.whmc.af.mil > -----Original Message----- > From: Maryalice Stetler-Stevenson [mailto:stetler@box-s.nih.gov] > Sent: Tuesday, February 13, 2001 8:19 AM > To: cyto-inbox > Subject: Re: HCL variant vs. ? > This is an excellent example of variant hairy cell leukemia. The > strong > CD11c and 103 with bright CD22 and CD20 make the diagnosis. > Depending on who > you believe, CD103 is (according to my Hemepath gods) more specific > than > TRAP- which is positive in a number of NHL's. CD10 is found in some > HCL. The > dry tap goes with it. Was there a monocytopenia? > Maryalice > A 64year old gentleman with pancytopenia (WBC: 2 K/uL, Hbg:8.0g%, > Plt: > 80,000), found on a routine blood count. No infections, no > splenomegaly, > "healthy". He comes from Chernoble and works as a carpenter. Bone > marrow > aspirates twice were dry taps so a sample of peripheral blood was > sent for > phenotyping. 20% of his lymphocytes were strongly positive for > CD103, CD22, > CD19, CD20, Lambda, CD11c and CD10 with only about 30% of the cells > showing > weak to moderate staining for FMC7 They were negative for CD25 and > CD38. > TRAP is positive in these cells but morphologically they are not > quite > classical hairy cells. Can this be a HCL variant? Has anyone seen a > lymphoma > with monoclonal B cells strongly positive for CD103 and CD11c ? > Judy > Judith Chezar > Head Hematology and Blood Bank laboratories > Hospital of Western Galilee > Nahariya, Israel > Maryalice Stetler-Stevenson > Director Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH > "Learn the rules so you know how to break them properly." > The Dalai Lama > > Maryalice Stetler-Stevenson > Director Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH > "Learn the rules so you know how to break them properly." > The Dalai Lama
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