See the February issue of Nature Medicine, De Rosa et al., for a reasonably thorough analysis of exactly this question. Briefly, CD62L (in human) is awful by itself. CD45RA is pretty bad, giving about 95% purity in CD4 but typically only about 50% among CD8 (and that's for healthy adults; for HIV+, these numbers drop dramatically). You have to use the two in combination (or CD45RA plus any number of other markers) to do reasonably well. mr At 9:33 AM -0500 2/15/01, Sharon Shalekoff wrote: >Hi > >Thanks to everyone for the replies to my quantitation question. > >To what extent does CD62L define naive cells as apposed to >CD45RA. If you gate on CD45RA, what proportion of those cells >will be CD62L positive and vise versa? > >Thanks again. >Sharon >Sharon Shalekoff >P/Bag X4 >Sandringham >2131 >South Africa > >sharons@niv.ac.za
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:06 EST