Antigen specific assay

From: MAINA BHAMAN (m_bhaman@chimeric.com)
Date: Mon Feb 12 2001 - 13:12:44 EST


Hi, we are trying to look at intracellualr staining after antigen specific activation of lymphocytes . Specifically we are trying to activate with CMV protein gp65 .. anybody with any experience with this . We can't seem to get much activation or cytokine production as compared to unstimulated and SEB positive controls.

Would appreciate any help we can get
Thanks
Maina Bhaman
Chimeric Therapies Inc.



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