Derek, We do it often. Is it necessary? . . . only if you feel that viability may effect your results. Non-specific increases in antibody binding will often be seen on dead cells. And, using some sort of viability marker is certainly better than simply using light scatter. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Flow Core 7416 CCGC 0946 (734) 647-3216, fax (734) 936-7376 kukuru@umich.edu >>> Derek Schulze <flow@post.queensu.ca> 02/07/01 04:56PM >>> As a general question, how many people out there are using PI to gate out dead cells when immunophenotyping? Is this really necessary? Derek Schulze Cancer Research Labs Queen's University 353 Botterell Hall Kingston, ON, K7L 3N6 (613) 533-6635 http://meds.queensu.ca/medicine/crl/
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